Friday 28 February 2020

Lupine publishers|Climate Resilient Interventions for Improving Food Security in Hill Ecology of Hindu Kush Himalaya

Lupine publishers|open access environment journals

Abstract

The Hindu Kush Himalaya (HKH) region has significant implications for the agro based economies of eight adjoining countries, because of their large dependence on irrigation water originating in the HKH region. However, the HKH region is extremely vulnerable to climate change impacts, which is evident through frequent floods, droughts and retreating glaciers, thus leaving negative consequences for agriculture and livelihood. It is also evident that the traditional farming methods are no longer sustainable to cope with the changing climatic conditions for sustainable production in hill ecology. Therefore, innovative farming technique and irrigation technologies needs to be adopted for mitigating climate change impacts on agriculture and for ensuring food security. Although some pioneer work has been done in developing and promoting improved farming methods and irrigation techniques, but these improvements were mainly focused in major leveled irrigated areas lying downstream of HKH region. Thus, investment on promoting site specific climate resilient practices in hill ecology of HKH was largely overlooked. Addressing these issues, this study is focused on assessment of agricultural production system of Pakistan, as a case example by having 51% area falling in HKH region and reviewed few promising interventions/technologies successfully adopted in downstream irrigated areas that may be equally beneficial for the hill ecology of HKH region. Anecdotal evidence indicates that promotion of these interventions will improve community resilience to climate change and may lead to increased agricultural production and better livelihood for hill ecology of HKH region. This may also improve sustainable irrigation water supply for the irrigated areas downstream.
Keywords:Irrigation Technology; Sprinkler System; Drip System; Furrow Bed; Climate Change; Small Farmers; Water Use Efficiency

Introduction

The total area of Hindu Kush Himalaya (HKH) region is ~ 3.44 million sq. km spread over eight countries of Asia. The 51% area of Pakistan (~0.4 million sq. km) falls in HKH region, which form around 12% part of the whole HKH region [1]. Pakistan is home to one quarter (~19%) of the total population (~211 million people) of HKH region with an average population density of 97 persons per sq. km. The second (K2) and ninth (Nanga Parbath) highest peaks of HKH region fall in Pakistan. The agro based economy of HKH region in Pakistan is heavily dependent on the production of vegetables, crops and fruits. Current climate change trends and lack of adequate integrated water resource management strategies are causing huge losses to the country due to frequent droughts and floods [2]. Therefore, traditional farming techniques prevalent in Pakistan are no longer capable to keep up with the rapid climate change variability in the HKH region [3,4] and its downstream basins.
The Indus Basin of Pakistan is comprised of one of the largest contiguous canal irrigation systems in the world that lies at the downstream of HKH, thus prone to frequent climatic disasters [5]. For instance, the recent 2010 floods, originated in HKH region, drowned around one fifth of Pakistan, affected ~20 million people and caused more than US $43 billion impact on the country’s economy [6]. Irrigated agriculture produces around 90% of crop production in Pakistan and contributes more than 21% in the country’s GDP. Moreover, production in Pakistan has global implications, thus may impact on global food security. For instance, Pakistan produces wheat crop greater than the whole Africa and nearly equal to South America. Therefore, agriculture needs to be more wisely managed and traditional farming and irrigation methods needs to be modified according to the changing climatic conditions. This paper reviews some of the salient features of agriculture in Pakistan, its vulnerability to climate change and few promising irrigation technologies capable of improving farming system resilience to climate change.

Salient Features of Pakistan’s Agriculture and their Vulnerability to Climate Change

Land use in Pakistan

The province wise land use is presented in Table 1. The total area of Pakistan is 79.61 million hectares (mha) with 23.40 mha cropped area. The current cultivated area is concentrated in Punjab province with 12.46 mha, which is ~50 % of the total cropped area of Pakistan followed by Sindh Province at ~8 % of country’s cropped area. However, there is 7.82 mha culturable waste area that is largely concentrated in least developed provinces of Khyber Pakhtunkhwa (KP) and Baluchistan, which can be brought under cultivation to enhance food security. The province wise culturable waste areas are 12%, 23%, 64% and 190% of current cultivated lands in Punjab, Sindh, KP and Baluchistan respectively. Therefore, the agro based economies of KP and Baluchistan has greater potential for improvement by cultivating their culturable waste lands.
Table 1: Land use in Pakistan.
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The irrigated cropped area in Pakistan is around 18.84 mha (Table 2) according to Agriculture Statistics of Pakistan 2006-07. This includes: wheat 7.34 mha (36.3 % of total); rice 2.58 mha (12.8 % of total); cotton 3.01 mha (15.2 % of total); sugarcane 1.03 mha (5.1 % of total); and fodders 2.00 mha (9.9 % of total). These five crops cover 16.03 mha of total irrigated area in Pakistan, which is 79.32% of the total irrigated area. Therefore, improving water productivity of these five major crops would have major impacts on productivity of irrigated agriculture in Pakistan. Other crops cover 4.17 mha; which constitute around 21.68 % of total irrigated area in Pakistan. The climate change induced floods and droughts significantly impact on crop production, especially during Kharif (summer) season, from both irrigated and rain-fed areas of Pakistan. The current land use methods tend to exacerbate the soil and water degradation and reduce land productivity potential. Similarly damage to standing crops due to lodging caused by heavy winds, water logging and salinity and frequent water stresses at crop critical growth stages causes production losses. Therefore, the traditional farming techniques need to be modified to control crop damage due to climate change induced threats, which is essential for sustainable agriculture in the country.
Table 2: Irrigated cropped area of major crops in Pakistan.
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Temporal Water Availability During Rabi and Kharif Seasons In Pakistan

Temporal surface water availability and deficiency during rabi (winter) and kharif (summer) seasons in Pakistan is shown in Figure 1. Temporal changes indicated up to 23% increase and down to 16% decrease in total annual available surface water since 2001 until 2007. However, the variability in total annual available surface water diminished below 5% since 2009 onward. Interestingly a major flood occurred during 2010, but data show insignificant impact on the total water availability (Figure 1). Thus, climate change cannot be judged from the total water availability. However, the frequency, intensity and distribution of rainfall are important parameters to be considered. Similarly, the average deficiency remained around 6.4% during Kharif (summer season) and around 24% during Rabi (winter season) with an average annual water deficiency of around 13% during the last twelve years.
Figure 1: Temporal surface water availability and deficiency during rabi (winter) and kharif (summer) seasons in Pakistan.
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Increased Use of Groundwater in Pakistan

Canal water fluctuation and unreliability due to climate change has increased farming community dependence on groundwater during the last decade. The changing trend of water availability is presented in Table 3. The statistics of canal and tube well irrigated area in provinces from 1993-94 to 2010-2011 (Table 3) indicate a decrease of 19% and 26 % in canal irrigated areas and an increase of 26 % and 157% in tube well irrigated areas in Punjab and Sindh provinces respectively. The overall tendency from 1993-94 to 2010- 11 indicated a decrease of 17.5 % in the canal irrigated areas and an increase of 33.5 % in groundwater irrigated areas in Pakistan. The overexploitation of groundwater is coupled with saline water up-coning and disposing of deep brackish groundwater into the soil surface thus causing fertile lands barren in Pakistan. It is therefore essential to reduce groundwater usage by utilizing surface water more efficiently for fulfilling irrigation demands of larger areas. Thus, the traditional irrigation methods need to be modified to more efficient methods to reduce groundwater usage.
Table 3: Temporal change in irrigated area under surface and groundwater in Pakistan.
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Climate Resilient Interventions for Improving Food Security in HKH Region

The existing land use, crops, water availability and usage statistics clearly indicate that the traditional farming techniques are not capable to cope with the climate change induced risks. It is therefore essential to adopt suitable climate change risks mitigation interventions for improving food security in HKH region. Few of these interventions/technologies successfully adopted in irrigated leveled lands downstream of HKH are summarized below:

Furrow Bed (Raised Bed) Irrigation Systems

Furrow bed irrigation system is one of the commonly used form of surface irrigation throughout the world (Figure 2) and is generally considered a more water-efficient system compared with the traditional flat basin because of (i) the speed with which water is conveyed to the low end of a field [7]; and (ii) the relatively small proportion of the soil surface is in contact with the flowing water during irrigation than the basin [8]. Furrow bed irrigation system is important in the current climate change scenario of
Figure 2: Advantages of furrow bed over flat basin [11].
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Pakistan agriculture in particular and Himalaya region in general due to the following reasons:
a) Furrow bed can save up to 50% irrigation water in comparison with traditional flat basin thus can reduce impact of limited water availability on crops in drought prone areas;
b) Furrows can quickly drain excess floodwater thus can reduce crop damage in flood prone areas;
c) Furrow beds reduce crops lodging by providing safe path and exit to speedy wind currents and greater reinforcement due to strong roots and soil grip;
d) Furrow beds laid perpendicular to slope can increase soil infiltration and ground water recharge by delaying runoff down slope and providing larger surface water storage capacity in furrows;
e) Conservation agricultural practices including minimum tillage, ground cover/mulching etc can be conveniently adopted on furrow beds which reduce soil erosion and soil and water degradation;
f) Furrow beds support mechanized farming thus can increase crop production and can be adapted to technological progress.
The Climate, Energy and Water Research Institute (CEWRI) of Pakistan Agricultural Research Council (PARC) has played a pioneer role in evaluation of furrow beds in Pakistan. Research conducted has shown that furrow beds is effective in improving yield of wheat by 20%, cotton 19%, Maize 58%, rice 26% compared with flat basin or ridge irrigation systems [9,10]. Similarly, crop yield per unit area increases by increasing the bed width. For instance, crop yield of maize showed up to 15% increase while wheat crop showed 26% increase on 180 cm bed size compared with flat basin [11], which was the largest increase noted when compared with other bed sizes given in Figure 2. Water saving of up to 50% in wheat, 30% in maize, 40% in cotton, 29% in rice were reported by N Ahmad et al. [9], Gill et al. [10], Hassan et al. [12]. Increase in water saving by increasing bed has been demonstrated in Figure 2 [9], which shows up to 40% and 36% increase in water saving by adopting 180cm bed size for maize and wheat crops respectively. Furrow bed increase crop yield and reduce irrigation application thus consequently crop water productivity is increased. For instance, an increase in water productivity of up to 70% in maize and up to 43% in wheat were reported by Hassan et al. [12] and Akbar et al. [13]. Impact of bed width on water productivity is illustrated in Figure 2, which shows up to 70% and 43% increase in water productivity for maize and wheat crops respectively compared with flat basin [13].
The CEWRI-PARC in collaboration with Australian Centre for International Agricultural Research (ACIAR) played a pioneer role in adopting furrow bed irrigation system in Maize wheat system of Pakistan. Conservation agricultural practices involving minimum soil disturbance were introduced in the form of permanent raised bed (PRB) farming system. Different bed width and furrow sizes were evaluated and demonstrated at farmer fields. Raised bed machinery imported from Australia was indigenously produced. The furrow bed farming system was disseminated by giving incentive to the farmers in the form of giving subsidies in purchasing raised bed machinery, training and demonstrations in the country. Although there are still issues in the machinery, especially for adopting PRB farming system, but the furrow bed system has been adopted widely for maize, cotton, sugar beat and vegetables crops throughout the country.

Sprinkler Irrigation Systems

In sprinkle irrigation method water is spayed into the air at 70-700 kpa (10-100 psi) pressure that fall on the ground or crop canopy like rainfall [14]. Using sprinkle irrigation system, the crop requirement can be accurately fulfilled through a combination of measures involving careful selection of sprinkle nozzle size, operating pressure and sprinkle spacing, thus adjusting sprinkle application rate to suit the soil infiltration rate. Sprinkle irrigation systems have increased importance for HKH region in the current climate change scenario due to the following reasons:
a) Use limited available water more efficiently as the application efficiency of a well-designed sprinkle system can vary from 60-80% thus can increase crop production and irrigated lands;
b) No land leveling required thus reduce operation cost and protect environment by avoiding cutting and filling of cultivated lands;
c) Irrigation of steep and rolling topography without producing runoff or erosion;
d) Greater potential to increase agricultural production by bringing extra undulating and sloppy HKH terrain under irrigation;
e) Light shower can avoid crop damage due to temperature extremes year around;
f) Timely irrigation of few centimeter using sprinkler irrigation system can double crop yield at critical crop growth stages, especially during wheat germination in HKH region;
g) The pre-sowing irrigation (Rouni) can be applied with sprinkler system for timely planting of crops.
All these factors increase sprinkle irrigation system suitability to the HKH region in the current climate change scenario. However, there are few drawbacks. For instance, these systems are affected by wind and, depending on the size of droplets and the spray trajectory, uniform distribution may be limited. Sprinkle systems have high initial costs and maintenance requirements. They also use high operating pressures, which has large energy requirement. The cost of portable and semi portable system is less but difficult to operate due to movement of laterals. The CEWRI-PARC in collaboration with local industries developed a complete range of rain gun sprinkler irrigation systems, including diesel, electric and PTO-driven pumping systems, couplers, other fittings and joints. To keep the systems portable, the pumping unit along with power unit (diesel engine, electric motor and solar panels) was mounted on a trolley. These systems have been used in the Khanpur Dam area where the rain gun sprinkler irrigation system is being used for establishment of orchards, efficient irrigation of fruits and vegetables, washing of Lychi and citrus, and for cooling and frost control in orchards. Initial investment requirements and unreliable electricity are restricting wider adoption of this technology.
These rain gun sprinkler irrigation systems have been installed in various parts of Pakistan for demonstration and introduction of the technology. In rain fed areas, these systems are being used for supplemental and life-saving irrigations to fruits, vegetables and field crops. The research conducted by CEWRI-PARC resulted in local fabrication of Poly Ethylene (PE) based irrigation system components, sprinklers and high-pressure pumps. Based on research findings and success of pilot-scale installations, the Government of Pakistan launched a National Program entitled “Water Conservation and Productivity Enhancement through High Efficiency Irrigation Systems in Pakistan” during 2007-12. Drip and sprinkler irrigation systems were installed on an area of 6852 acres in all provinces (PARC 2010). The major area was in Punjab and followed by KP. There were 255 farmers benefitted from the project. There were 80 % sprinkler irrigation systems and 20 % were drip system during the period (Table 4).
Table 4: Drip and sprinkler irrigation systems in provinces, installed under the national project (from 2007-2012).
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Drip Irrigation Systems

Drip irrigation system applies water close to each plant and the application uniformity depends on the uniformity of discharge from the emitters [14]. It generally comprises of a pumping station, control head, main and sub main lines, lateral lines, emitters, valves and fittings. The drip irrigation system has special agronomical, agro technical and economic advantages that make it a suitable option of irrigation in the HKH and adjoining region. These advantages are as under:
a) Drip irrigation can conveniently and efficiently supply water directly to the individual crop rows or plants, thus can effectively utilize small continuous streams of water in the HKH region;
b) It reduces water requirement by saving up to 50% irrigation water to a young orchard as compared to sprinkle or surface irrigation methods thus can enhance community resilience to drought;
c) It requires less labor, as a well-regulated system can effectively utilize a continuous stream of water, a norm in HKH region, with less farmer supervision thus can increase production;
d) It discourages weeds growth and offers greater control over fertilizer placement and timing thus can save farmer resources;
e) Drip irrigation can be designed for any topography even if the area is rocky with steep slopes and plants are with irregular spacing, which increase its importance for HKH region.
Drip irrigation systems have shown yield gains of up to 100%, water savings of up to 40–80%, and associated fertilizer, pesticide, and labor savings over conventional irrigation systems [15]. The low rate of water application reduces deep percolation losses. The systems have lower energy requirements than sprinkler systems because of lower operating pressure requirement. The water application uniformity of locally developed drip irrigation system was above 85 % [16]. Evaluation of drip systems at CEWRIPARC showed lowest coefficient of variation of locally developed microtube emitters [17] with application uniformity above 85 per cent [16]. This shows that trickle irrigation systems have potential to use scarce water resource more efficiently if designed properly. The evaluations by Ahmad MM et al. [18] indicated that drip system with micro tube emitters can be operated at low pressure head (3.5 m) with an insignificant loss in uniformity as compared to operating at high pressure head (10 m), which can save energy cost, as given in Table 5. This low head drip system is being used for small scale vegetable production in Pakistan.
Table 5: Microtube emitter discharge and water application uniformity at various pressure heads and distances Ahmad et al. [9].
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These research outcomes are currently being disseminated through training and demonstration in provinces. The provincial governments are adopting drip/sprinkler systems evidenced through various programs, indicating government commitment for accelerating drip/sprinkler system adoption. By successful adoption of these technologies, more water can be saved or efficiently used along with an increase in yield. The saved water will automatically reduce over exploitation of groundwater and consequently mitigating the secondary salinity by improving overall water use efficiency of the irrigation system. This shows that the system has potential to use scarce water resource more efficiently if designed properly.

Conclusion and Recommendations

a) Lack of adoption of climate change resilient practices in the hill ecology of HKH region is negatively affecting their food security and also have negative implications for the irrigated agriculture downstream in adjoining countries;
b) The 51% area of Pakistan falls in HKH region, thus was considered as a representative case example for analyzing their agricultural production system, growing food security and climate change issues and promising climate resilient interventions mainly adopted in downstream leveled irrigated areas of HKH region;
c) Furrow bed, sprinkler and drip irrigation systems has been shown efficient irrigation methods with multiple advantages thus can be instrumental for improving community resilience to climate change by conserving the declining and uncertain available water resources in the hill ecology of HKH region;
d) However, adoption of these technologies is equally important for improving climate change resilience, food security and livelihood of the hill ecology of HKH region.


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Lupine Publishers: Panduan Penternakan Burung Puyuh (Malay Version)

Lupine Publishers: Panduan Penternakan Burung Puyuh (Malay Version): Lupine Publishers- Environmental and Soil Science Journal Opinion Written by Jabatan Perkhidmatan Veterinar Neg...

Thursday 13 February 2020

Wednesday 12 February 2020

Effect of Interaction Between Ag Nanoparticles and Salinity on Germination Stages of Lathyrus Sativus L.

Lupine publishers|Effect of Interaction Between Ag Nanoparticles and Salinity on Germination Stages of Lathyrus Sativus L.

 Lupine publishers|Earth and environment journals

Abstract


The aim of the study was to effect of interaction between Ag nanoparticles and salinity on Germination Stages of Lathyrus Sativus L. Treatments included in the study were viz. To 3 levels of salinity (0 as control, 8 and 16 dS/m NaCl), 8 and 16 dS/m and four levels of silver nanoparticles (0, 5, 10 and 15 ppm) on grass pea seed were tested. An experiment was conducted to evaluate the effects of silver nanoparticles (AgNPs), on the seed germination factors, root and shoot length (RL and SL) and proline content of grass pea Survival under Salinity Levels. Results showed a significant reduction in growth and development indices due to the salinity stress. The salt stress impaired the germination factors of grass pea seedlings. The application of Ag in combination improved the germination percentage, shoot and root length, seedling fresh weight and seedling dry weight and seedling dry contents of grass pea seedlings under stressed conditions. The results suggest that Ag nanoparticles enhancement may be important for osmotic adjustment in grass pea under salinity stress and application of Ag mitigated the adverse effect of salinity and toxic effects of salinity stress on grass pea seedlings.
Keywords: Ag nanoparticles; Salinity; Germination Stages; Grass Pea; Lathyrus Sativus L.

Introduction

High salinity is a common abiotic stress factor that causes a significant reduction in growth. Germination and seedling growth are reduced in saline soils with varying responses for species and cultivars [1]. Soil saltiness may impact the germination of seeds either by causing an osmotic potential outside to the seed averting water uptake, or the poisonous effects of Na+ and Cl− ions on germinating seed [2]. Salt and osmotic stresses are responsible for both inhibition or delayed seed germination and seedling establishment [3]. The majority of our present-day crops are adversely affected by salinity stress [4]. NaCl causes extensive oxidative damage in different legumes, resulting in significant reduction of different growth parameters, seed nutritional quality, and nodulation [5,6]. To mitigate and repair damages triggered by oxidative stress, plants evolved a series of both enzymatic as well as a non-enzymatic antioxidant defense mechanism. Ascorbate and carotenoids are two important non-enzymatic defenses against salinity, whereas proline is the most debated osmoregulatory substances under stress [7].
Lathyrus Sativus L. (Grass pea) is an annual pulse crop belonging to the Fabaceae family and Vicieae tribe [8]. Grass pea has a long history in agriculture. The crop is an excellent fodder with its reliable yield and high protein content. This plant is also commonly grown for animal feed and as forage. The grass pea is endowed with many properties that combine to make it an attractive food crop in drought-stricken, rain-fed areas where soil quality is poor and extreme environmental conditions prevail [9]. Despite its tolerance to drought it is not affected by excessive rainfall and can be grown on land subject to flooding [10,11]. Compared to other legumes, it is also resistant to many insect pests [12-15]. Nanoparticles (NPs) are wide class of materials that include particulate substances, which have one dimension less than 100 nm at least [16]. The importance of these materials realized when researchers found that size can influence the physiochemical properties of a substance e.g. the optical properties [17]. NPs with different composition, size, and concentration, physical/ chemical properties have been reported to influence growth and development of various plant species with both positive and negative effects [18]. Silver nanoparticles have been implicated in agriculture for improving crops. There are many reports indicating that appropriate concentrations of AgNPs play an important role in plant growth [19,20]. The application of Nano silver during germination process may enhance germination traits, plant growth and resistance to salinity conditions in basil seedlings [21]. The use of Silver Nanoparticle on Fenugreek Seed Germination under Salinity Levels is a recent practice studied [22]. Nanomaterials have also been used for various fundamental and practical applications [23]. Although the potential of AgNPs in improving salinity resistance has been reported in several plant species [24,25], its role in the alleviation of salinity effect and related mechanisms is still unknown. Therefore, the main objective of this work was to study the effect of Silver Nanoparticles on salt tolerance in Lathyrus Sativus L.

Material and Methods

In order to investigate salinity stress on Lathyrus Sativus L. germination indices, an experiment was carried out in Iran from April to Juan 2017 at Ferdowsi University of Mashhad, to creation salinity, sodium chloride at the levels of 8 dS/m, 15 dS/m and 0 (as control), four levels of silver nanoparticles (0, 5, 10 and 15 ppm) on Grass pea were tested. The Ag NPs were obtained from US Research Nanomaterial’s, Inc. Transmission electron microscopy (ТЕМ) images of silver nanoparticles with diameters of 20 nm, shown in Figure 1. Seeds of Lathyrus Sativus L. where from seed bank of Research Center for Plant Sciences, Ferdowsi University of Mashhad. These all were washed with deionized water. Seeds were sterilized in a 5% sodium hypochlorite solution for 10 minutes [26], rinsed through with deionized water several times. Their germination was conducted on water porous paper support in Petri dishes (25 seed per dish) at the controlled temperature of 25 ± 1°C. After labeling the Petri dishes, seed were established between two Whatman No. 2 in Petri dishes. Silver nanoparticles in different concentration silver nanoparticles (0, 5, 10 and 15ppm) were prepared directly in deionized water and dispersed by ultrasonic vibration for one hour. Each concentration was prepared in three replicates. Every other day supply with 0.5 ml silver nanoparticles per every test plantlet was carried out for 21 days along with control. Germination counts were recorded at 2 days’ intervals for 21 days after sowing and the seedlings were allowed to grow. The germination percentages of the seeds were finally determined for each of the treatments. After 21 days of growth, the shoot and root lengths were long enough to measure using a ruler. The controlled sets for germinations were also carried out at the same time along with treated seeds (Figure 2).
Figure 1: Silver Nanopowder, Coated with ~0.2wt% PVP (Poly Viny Pyrrolidone) surfactant for low oxygen content and easy dispersing. True density: 10.5 g/cm3 Purity: 99.99% APS: 20 nm SSA: ~18-22 m2/g Color: black, Morphology: spherical.
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Figure 2: Effect of Ag Nanoparticles on Germination Stages of Lathyrus Sativus L. in Salinity level( 8 dS/m NaCl).
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Parameters Measured in this Study were:

A. Germination Stages
Total germination percentage (GT) was calculated as Gt = (n/N ×100), where n = total number of germinated seeds (normal and abnormal) at the end of the experiment and N = total number of seeds used for the germination test.
B. Germination Speed Index (GSI)
Conducted concomitantly with the germination test, with a daily calculation of the number of seeds that presented protrusion of primary root with length ≥2 millimeter, continuously at the same time amid the trial. The germination speed index was calculated by Maguire formula [27]: aguire formula (1962):

Where:
GSI = seedlings’ germination speed index;
G = number of seeds germinated each day;
N = number of days elapsed from the seeding until the last count.

Root and Shoot Length

Root length was taken from the point below the hypocotyls to the end of the tip of the root. Shoot length was measured from the base of the root- hypocotyl transition zone up to the base of the cotyledons. The root and shoot length were measured with the help of a thread and scale.

Seedling Vigour Index

The seedling vigor index was determined by using the formula given by Abdul baki and Anderson [28].

Fresh and Dry mass

The fresh mass was quantified through weighing on precision scale, and the dry mass was determined through weighing on a precision scale after permanence of the material in a kiln with air forced circulation, at a temperature of 70°C, until indelible weight. At the ending of the experiment, At the end of the experiment, radical and plumule length and fresh weight measured. Plants were placed in the oven at 70°C for 48 h and weighted with sensitive scale.

Proline Contents

Proline was determined spectrophotometrically following the ninhydrin method described, using L-proline as a standard [29]. Approximately 300 mg of dry tissue was homogenized in 10ml of 3% (w/v) aqueous sulphosalicylic acid and filtered. To 2ml of the filtrate, 2ml of acid ninhydrin was added, followed by the addition of 2ml of glacial acetic acid and boiling for 60 min. The mixture was extracted with toluene, and the free proline was quantified spectrophotometrically at 520nm from the organic phase using a spectrophotometer. Statistical analysis each treatment was conducted, and the results were presented as mean ± SD (standard deviation). The results were analyzed by one-way ANOVA with used Minitab Version 16.

Results and Discussion

The present study showed clearly that salinity had a negative effect on the yield and its components of grass pea. It is well known that seed germination provides a suitable foundation for plant growth, development, and yield [30]. Increased salt concentration caused a decrease in germination percent (Table 1). Seed germination decreased as the doses increased. The Strong reduction in germination (-47%) was observed mainly at the highest level of salt concentration as compared to control treatment. Delayed germination causes increased irrigation cost and irregular and weak seedling growth in the establishment of legume crops. Relevant results were reported by Gunjaca and Sarcevic [31] and Almansouri et al. [32]. They reported that increasing osmotic potential decreased water uptake and slow down germination time. The average time of germination increases with increasing levels of salinity. In view of mean germination time, there was a considerable increase in this character at 0 (as control), 8 and 16 DS/m salinity levels as compared to the others. Emergence was significantly affected by salinity levels. Moreover, many researchers have reported developmental delay of seed germination at high salinity [33]. The germination rate decreased as salt concentration increased to a 16 dS/m and delayed for the high salt dosage (Table 1). Since higher salinity limited water absorption, it has prevented nutrient assimilation, as a result, germination rate declined with increasing salinity. The findings from this study were like to the findings of Kaydan and Yagmur [34] and Akhtar and Hussain [35].
Table 1: The interaction effect of NaCl and AgNPs on Germination Speed Index.
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Table 2: Analysis of variance of the measured traits.
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Shoot fresh weight was significantly influenced (P<0.05) by salinity levels. The highest shoot fresh weight was obtained from 0dSm salinity level while the lowest weight was at 16dSm. Shoot fresh weight significantly decreased as salinity level increased above 8dSm (Table 2). Salinity stress significantly (P<0.05) affected shoot dry weight as the salt concentration dosage increased. Shoot dry weight significantly decreased in salt levels over 8dSm. When the salinity level was raised above, the proline content increased in grass pea. Culturing excised roots has demonstrated to be a really great test show for the early detection of tolerance to abiotic stresses such as saltiness [36-38].
Proline was studied in numerous works dealing with plant selection against abiotic stresses such as dry and salinity [39,40], and it may play a defensive part against the osmotic potential produced by salt [41,42]. The proline substance of the expanded with the NaCl concentration of the culture medium. At 16 dS/m NaCl, the proline concentration appeared a huge increment in reaction to salt stress, although the activity of the roots at this concentration was negligible, with no grateful longitudinal development. Proline, which happens broadly in higher plants and collects in bigger sums than other amino acids [43], regulates the aggregation of useable N. Proline collection normally occurs within the cytosol where it contributes significantly to the cytoplasmic osmotic alteration [44]. It is osmotically very active and contributes to membrane stability and mitigates the impact of on NaCl cell membrane disturbance [45]. In the present experiment application of Ag NPs enhanced seed potential by increasing the characteristics of seed germination (Tables 1 & 2). The results showed that the impact of Ag NPs was significant on germination percentage in P≤0. 05. The results about of this test appeared that utilization of Ag NPs nanoparticles can increment the germination in grass pea. Seed germination results indicate that Ag Nanoparticles at their lower concentrations advanced seed germination and early seedling growth in grass pea, anyway at higher concentration showed slight antagonistic impacts. Parameters of seed germination were expanded with increasing levels of Ag NPs up to 10 ppm. Among the treatments, application of 10 ppm of Ag NPs proved best by giving the highest values for percent seed germination, germination rate and germination mean time. It is well watched that the exogenous application of Ag NPs decreased the reduction of germination resulted from salt treatments. In the interim, the control treatments of salt and Ag nanoparticles gave the tallest plants contrasted with the other studied treatments. Darvishzadeh et al. [21] found that the utilization of Ag Nano particles at the concentration of 40 mg.kg-1 prompted the increases in germination percentage and improved the resistance to salinity conditions in basil. The proline content increased with increasing severity of salinity stress. Additionally, proline content significantly (P.0.01) increased when silver nanoparticles were applied in connected in serious saline stress in comparison without silver nanoparticles (Figure 3).
Figure 3: Effect of interaction between ag nanoparticles and salinity on germination stages of Lathyrus Sativus L. a - Main effects plot for Root length (mm); b - Main effects plot for dry weight of shoot and root (gr); c - Interaction Plot for fresh weight of shoot and root (gr); d - Interaction Plot for dry weight of shoot and root (gr); e - Interaction Plot for Proline (mg/gr).
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Conclusion

Salt stress through enhancement of osmotic pressure leads to the decrease of germination percentage, germination rate, germination index and an increment in mean germination time of Lathyrus sativus seeds. For overcoming the negative impacts of salinity on the plant growth and yield can be to attempt to new strategies. The dry and fresh weight of seedlings diminished as seedling length declined with increasing salinity levels since root number, shoot number, root length and shoot length decreased essentially. Results demonstrate that Ag NPs at lower concentration enhances seed germination, promptness index, and seedling growth. The positive effect of Ag on physiological properties was in conditions that the plant grew under salt stress was more increasingly exceptional in examination with the conditions that plant grown under normal conditions. The results of this study showed that Ag can be involved in the metabolic or physiological activity in higher plants exposed to abiotic stresses.

Acknowledgment

I would like to thank the Research Center for Plant Sciences, and Dr. Jafar Nabati for providing the necessary facilities.

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Soil and Environment

  Mini Review Soil is a blend of natural issue, minerals, gases, fluids, and life forms that together help life. Earth’s collectio...